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KMID : 0545120020120020279
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 2 p.279 ~ p.285
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Isolation of Phytase-Producing Pseudomonas sp. And Optimization of its Phytase Production
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Kim, Young Hoon
Gwon, Moon Nam/Yang, Si Yong/Park, Tae Kyu/Kim, Chan Gil/Kim, Chang Won/Song, Min Dong
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Abstract
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Phytase (myo-inositol hexakisphosphate phosphohydrolase, EC 3.1.3.8) catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate) to release inorganic phosphate. A bacterial strain producing phytase was isolated from soil around a cattle shed. To identify the strain, cellular fatty acids profiles, the GC contents, a quinine-type analysis, and physiological test using an API 20NE kit were carried out. The strain was identified to be a genus of Pseudomonas sp. and named as Pseudomonas sp. YH40. The optimum culture condition for the maximum productivity of phytase by Pseudomonas sp. YH40 were attained in a culture medium composed of 1.0% (w/v) glycerol, 2.0% (w/v) peptone, and 0.2% (w/v) FeSO_47H_2O. Within the optimal medium condition, the production of phytase became highest after 10h of incubation, and the maximal phytase production by Pseudomonas sp. YH40 was observed at 37℃ and pH 6.0.
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